Naveni® Plex Omni Service – The Future of Spatial Interactomics

Many of you will remember having to memorize numerous cellular signal transduction pathways during your studies – including, most likely, the MAP kinase pathway. This cascade serves as a central regulatory hub in human cells, branching into numerous downstream signaling routes and playing a key role in embryogenesis, cell differentiation, cell growth and programmed cell death [1, 2]. Upon ligand binding to the EGF receptor (EGFR), the G-protein Ras becomes activated and triggers a phosphorylation cascade involving three sequential kinases [1]. In addition to its fundamental physiological functions, the MAPK signaling pathway also harbors substantial oncogenic potential: mutations in the Ras proto-oncogene can cause constitutive receptor-independent activation of the Ras protein. This not only promotes tumorigenesis, but can also give rise to rare genetic disorders known as RASopathies [2].

Due to its high clinical relevance, mapping the MAPK signaling pathway is of great interest to many researchers – and our partner Navinci has now made this possible. With the new Naveni® Plex Omni Service, the entire cascade can be tracked, from the activation of EGFR all the way to the phosphorylation of ERK1, one of the MAP kinases [3]. And the Omni Service goes far beyond the MAPK pathway: the proprietary multiplex in situ Proximity Ligation Assay (misPLA) technology enables the simultaneous detection of up to eight protein-protein interactions, individual proteins or phosphorylation states within their spatial context – ranging from MAPK signaling to immune checkpoint inhibitors to T- and B-cell markers.

These topics await you:

1) Naveni® Plex Omni Service – The First isPLA-Based Multiplexing System

2) The Next Generation of Spatial Interactomics – Carried Out by Experts

3) Eight Steps to Your Results: How the Naveni® Plex Omni Service Works

Naveni® Plex Omni Service – The First isPLA-Based Multiplexing System

On September 30th, 2025, Navinci introduced the new Naveni® Plex Omni Service, giving researchers worldwide access to state-of-the-art spatial interactomics [4]. Using the innovative misPLA technology, protein function and molecular networks can be mapped directly within their spatial context. This provides valuable insights into tumor-stroma communication, immune cell states, and key molecular networks that shape therapy response [3]. The underlying technology is based on antibody-oligonucleotide pairs that generate an amplifiable DNA signal when proteins are in close proximity, thereby visualizing protein interactions and post-translational modifications (Fig. 1). Up to eight such pairs can be used simultaneously. When the respective target epitopes are located sufficiently close to one another, ligation and rolling-circle amplification are triggered, resulting in a strong fluorescence signal (Fig. 1) [5].

The Next Generation of Spatial Interactomics – Carried Out by Experts

The best part of the Naveni® Plex Omni Service? The assay is performed directly by the very experts who developed it. From consultation to data delivery, the entire process is handled by the Navinci team – meaning you can focus entirely on the scientific insights rather than on assay optimization. You may choose freely from 50 verified targets to assemble a customized 4- to 8-plex panel tailored to your research needs. Alternatively, Navinci also offers prevalidated “ready-to-use” panels, including MAPK, immuno-oncology, and TCR/BCR panels [3]. Once the panel has been defined, you simply send in your samples – and from that point onward, you can leave the hands-on work to the experts. Multiple sample types are supported, including FFPE sections and tissue microarrays, fresh-frozen tissue samples, and fixed cultured cells.

Eight Steps to Your Results: How the Naveni® Plex Omni Service Works

1. Panel design
   Configure your 8-plex in situ Multiplex Proximity Ligation Assay (isPLA) panel from a list of pre-validated targets. Please enter the Biomol referral code “NP-OMNI-BIOMOL” in the Omni Panel Builder (Fig. 2).
   
   
2. Study consultation
   Meet with the Omni Service team to discuss project scope, timelines, and image analysis options.
   
   
3. Quotation and terms
   You will receive a detailed quote along with all relevant information regarding service conditions.
   
   
4. Sample shipment
   Your samples are shipped to Navinci’s headquarters in Uppsala, Sweden, following the clearly defined shipping guidelines.
   
   
5. Assay execution
   The next-generation multiplex isPLA assay is performed on your samples directly by the developers of the technology.
   
   
6. Optional: Image analysis
   Upon request, image analysis can be performed by specialists at Propath UK.
   
   
7. Data delivery
   Your results are securely delivered to you via Pathcore Flow.
   
   
8. Completion and invoicing
   After project completion, you will receive the invoice – marking the official end of the service.

Ready for cutting-edge spatial interactomics? Create your own Naveni® Plex Omni Panel now using Navinci’s Omni Panel Builder. Want to learn more? Read the proof-of-concept paper on the misPLA technology in collaboration with Uppsala University and discover additional information in the Naveni® Plex Omni product flyer.

Sources

[1] https://de.wikipedia.org/wiki/MAP-Kinase-Weg, 14.10.2025.

[2] https://flexikon.doccheck.com/de/Ras/Raf/MAPK-Signalweg, 14.10.2025.

[3] https://navinci.se/technology/naveni-plex/naveni-plex-omni-service/, 14.10.2025.

[4] https://navinci.se/naveni-plex-omni-service-launch/, 14.10.2025.

[5] Löf, L., Xu, B., Sinha, T.K., Dahlström, C., Vennberg, J., Larsson Forssén, T., Klaesson, A., Clausson, C.M., Wang, X., Strömberg-Olsson, U., et al. (2025). Spatial mapping of proteins and their activity states in cancer models by multiplex in situ PLA. bioRxiv, 2025.2007.2011.662357.

Preview Image: https://navinci.se/technology/naveni-plex/naveni-plex-omni-service/