--- title: HMGB1 ELISA Kits From Arigo Biolaboratories description: HMGB1 has attracted the attention of researchers as it is associated with various pathologies such as cancer, arteriosclerosis, and nephritis. image: https://resources.biomol.com/hubfs/Imported_Blog_Media/HMGB1-Overview-2.jpg --- en EN DE [** My account ](https://www.biomol.com/account) ** Service/Help - [Contact](https://www.biomol.com/contact) - [Technical Service](https://www.biomol.com/technical-service) - [FAQ](https://www.biomol.com/faq) - [Shipping Costs](https://www.biomol.com/shipping-costs) - [Product Search Tips](https://www.biomol.com/product-search-tips) - [Distributors](https://www.biomol.com/distributors) - [Company History](https://www.biomol.com/company-history) - Cookie Settings - [** Menu ](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#offcanvas--left) - [**Search Tips ](https://www.biomol.com/product-search-tips) - [** Search ](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#show-hide--search) ** Search   - 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In the nucleus, HMGB1 acts as a DNA-binding protein for gene transcription, gene regulation, and DNA repair.[1](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#References) In the cytosol, HMGB1 induces autophagy by interacting with Beclin-1 which results in its dissociation from Bcl-2 and the subsequent induction of autophagy.[2](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#References) In the extracellular compartment, HMGB1 is passively released by necrotic cells and acts as a chemokine and DAMP (damage-associated molecular pattern) to trigger inflammatory responses.[3](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#References) HMGB1 is also secreted from activated dendritic cells and macrophages and functions as a cytokine. It acts as a ligand for RAGE, TLR2 and TLR4, thus activating their signal pathways and ensuing inflammatory responses.[4](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#References) The HMGB1 released from apoptotic cells is neutralized by reactive oxygen species (ROS)-dependent oxidation and thus promotes immunological tolerance.  ## HMGB1 in Disease HMGB1 has attracted the attention of both basic and clinical researchers as it is associated with various pathologies such as cancer, sepsis, arteriosclerosis, chronic rheumatoid arthritis, and nephritis.[5-7](https://resources.biomol.com/biomol-blog/hmgb1-elisa-kits-from-arigo-biolaboratories#References) HMGB1 is reported as a necrosis marker and an elevated level of HMGB1 is a good indicator of effective anticancer therapy. Extracellular HMGB1 on the other hand gained attention due to its association with cell proliferation, invasion and metastasis, angiogenesis, and immunosuppression. ## HMGB1 ELISA Kits Arigo Biolaboratories offers the most comprehensive ELISA kits to facilitate HMGB1 research as well as the development of anticancer drugs. These ELISA kits provide three advantages compared to ELISA kits from other manufacturers. ![HMGB1 ELISA](https://resources.biomol.com/hs-fs/hubfs/Imported_Blog_Media/ARG81310-96-2.jpg?width=400&name=ARG81310-96-2.jpg) ### Specific Highly specific for HMGB1 and do not cross react with HMGB2 ### Flexible Suitable for multiple samples from multiple species ### Easy and Fast Requires only two incubations and one wash step ## ELISA Kit Comparison | **Product** | **Reactivity** | **Sample Type** | | | | | | | --- | --- | --- | --- | --- | --- | --- | --- | |   | Human | Mouse | Rat | Plasma | Serum | Cell Culture Supernatant | Lysate | | [Human HMGB1 ELISA Kit](https://www.biomol.com/products/assay-kits/elisa-and-immunoassays/human-hmgb1-elisa-kit-arg81185.96) | X |   |   |  X |   | X |   | | [Mouse HMGB1 ELISA Kit](https://www.biomol.com/products/assay-kits/elisa-and-immunoassays/mouse-hmgb1-elisa-kit-arg81310.96) |   | X | X | X |   | X |  X | | [HMGB1 ELISA Kit](https://www.biomol.com/products/assay-kits/elisa-and-immunoassays/hmgb1-elisa-kit-arg81351.96) | X | X | X |   | X |   |   | [**All Products from Arigo Biolaboratories**](https://www.biomol.com/arigo/)   ## References 1. [Czura C.J., Wang H., Tracey K.J., Dual roles for HMGB1: DNA binding and cytokine, *Journal of Endotoxin Research* 7, 4 (2001)](https://doi.org/10.1177%2F09680519010070041401) 2. [Tang D. et al., Endogenous HMGB1 regulates autophagy, *JCB Article* 190, 881-892 (2010)](https://doi.org/10.1083/jcb.200911078). 3. [Cai J. et al., The Role of HMGB1 in Cardiovascular Biology: Danger Signals, Antioxidants & Redox Signaling 23, 1351-1369 (2015)](https://doi.org/10.1089/ars.2015.6408) 4. [Man Y. et al., HMGB1 Signals Through Troll-Like Receptor (TLR) 4 And TLR2, Shock 26, 174-179 (2006)](https://insights.ovid.com/crossref?an=00024382-200608000-00011) 5. [Pisetsky D.S., Erlandsson-Harris H., Andersson U., High-mobility group box 1 (HMGB1): an Alarming Mediating the Pathogenesis of rheumatic disease, Arthritis Res Ther; 10, 209 (2008)](https://doi.org/10.1186/ar2440) 6. [Alleva L. M.,  Budd A. C., Clark I. A., Systemic release of high mobility group box 1 protein during severe murine influenz, *J Immunol.*](https://doi.org/10.4049/jimmunol.181.2.1454)[ 181, 1454–1459 (2008).](https://doi.org/10.4049/jimmunol.181.2.1454) 7. [Campana L., Bosurgi L., Rovere-Querini P., HMGB1: a two-headed signal regulating tumor progression and immunity,* **Curr Opin Immunol.* 20, 518–523 (2008). ](https://doi.org/10.1016/j.coi.2008.04.012) ## You might also like... SEE MORE BLOGPOST ![HMGB1 – A Protein with Many Faces](https://resources.biomol.com/hs-fs/hubfs/Biomol/Blog%20Images/HMGB1_Preview.png?width=600&name=HMGB1_Preview.png) ##### HMGB1 – A Protein with Many Faces Natural killer cells have the remarkable ability to specifically recognize tumor cells and eliminate them by releasing a deadly cocktail of... - [ELISA](https://resources.biomol.com/biomol-blog/tag/elisa) - [Antibodies](https://resources.biomol.com/biomol-blog/tag/antibodies) - [Proteins](https://resources.biomol.com/biomol-blog/tag/proteins) - [Cancer](https://resources.biomol.com/biomol-blog/tag/cancer) - [Arigo Biolaboratories](https://resources.biomol.com/biomol-blog/tag/arigo-biolaboratories) ![Wenmei Ma from Arigo Biolaboratories on Antibody Panels and Rabbit Monoclonals](https://resources.biomol.com/hs-fs/hubfs/Imported_Blog_Media/Arigo-AACR2019-2.jpg?width=600&name=Arigo-AACR2019-2.jpg) ##### Wenmei Ma from Arigo Biolaboratories on Antibody Panels and Rabbit Monoclonals What is your role at Arigo Biolaboratories and how long have you been with the company? I am the founder of Arigo Biolaboratories and the... - [AACR](https://resources.biomol.com/biomol-blog/tag/aacr) - [Interviews](https://resources.biomol.com/biomol-blog/tag/interviews) - [Arigo Biolaboratories](https://resources.biomol.com/biomol-blog/tag/arigo-biolaboratories) ![Inflammasomes - ](https://resources.biomol.com/hs-fs/hubfs/Imported_Blog_Media/NLRP3_Inflammasome_Structure_Preview-3.png?width=600&name=NLRP3_Inflammasome_Structure_Preview-3.png) ##### Inflammasomes - "Ignition Agents" of the Immune System AdipoGen Life Sciences combines immunology and metabolism and develops products for cancer, immunology, metabolic syndrome (diabetes,... - [Tips and Tricks](https://resources.biomol.com/biomol-blog/tag/tips-and-tricks) - [Antibodies](https://resources.biomol.com/biomol-blog/tag/antibodies) - [AdipoGen](https://resources.biomol.com/biomol-blog/tag/adipogen) - [Proteins](https://resources.biomol.com/biomol-blog/tag/proteins) - [Inflammation](https://resources.biomol.com/biomol-blog/tag/inflammation) - [Western Blot](https://resources.biomol.com/biomol-blog/tag/western-blot) - [Interleukins](https://resources.biomol.com/biomol-blog/tag/interleukins) Biomol Newsletter Subscribe to the free Biomol newsletter and receive updates on research tools from Biomol. 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